TIME-DEPENDENT EXPRESSION OF IL-1β mRNA - A PRELIMINARY STUDY FOR POSSIBLE WOUND AGE MARKER

  • Natasha Bitoljanu Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Ana Ivcheva Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Viktorija Belakaposka Srpanova Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Ljupco Cakar Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Rosica Siamkouri Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Aleksandar Stankov Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Goran Pavlovski Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Sasho Risteski Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
  • Zlatko Jakovski Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia

Abstract

Accurate estimation of wound age is a very important task in forensic medicine field, because it can helps in reconstruction of crime scene. IL-1β is proinflamatory cytokine and plays a major role in wound healing process. The aim of the study is to develop a method for the reliable estimation of dermal injury age. In this study the expression of IL-1β mRNA was evaluated by real-time PCR at skin human wounds with different post-injuried period. We collected 27 human skin wound samples from forensic autopsy cases at our Institute. In our study were include samples from lacerations, incised wounds and stab wounds, with a known time of injury and death. Wound samples were divided into five groups: control group (n=5); first group consisted of cases with immediate death (n=7); second group consisted of cases with survival from 1 hour to 6 hours (n=5); the third group comprised cases with survival from 6 hours to 72 hours (n=3); the fourth group comprised cases with survival from 72 hours to 168 hours (n=7). In this study we detected increased expression levels of IL-1 β mRNA in the second group with survival time from 1 to 6 hours.


Keywords: IL-1β; wound age; Real-time PCR; autopsy cases; human dermal injuries.


https://doi.org/10.55302/JMS2361121b


 

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Published
2023-05-10
How to Cite
BITOLJANU, Natasha et al. TIME-DEPENDENT EXPRESSION OF IL-1β mRNA - A PRELIMINARY STUDY FOR POSSIBLE WOUND AGE MARKER. Journal of Morphological Sciences, [S.l.], v. 6, n. 1, p. 121-126, may 2023. ISSN 2545-4706. Available at: <https://jms.mk/jms/article/view/vol6no1-16>. Date accessed: 27 feb. 2024.
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Articles