PROXIMAL RENAL TUBULES ENZYMURIA AS A SCREENING TEST IN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES

Dejan Spasovski; Emilija Sandevska; Svetlana Krstevska Balkanov; Ana Vaslievska; Ljindita Djemaili Jakupi; Elena Cvetinovska; Kujtim Iseini; Mediha Selimi; Vladimir Mitreski; Nikola Chamurovski; Maja Jakimovska Dimitrovska

Dejan Spasovski University Clinic for Rheumatology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
Emilija Sandevska University Clinic for Rheumatology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
Svetlana Krstevska Balkanov PHI University Clinic for hematology â€“Skopje, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
Ana Vaslievska University Clinic for Rheumatology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
Ljindita Djemaili Jakupi University Clinic for Rheumatology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
Elena Cvetinovska General City Hospital ;8th Septemberâ€™â€™ â€“ Skopje, North Macedonia
Kujtim Iseini General City Hospital -Tetovoâ€™â€“ Department of Internal Medicine, North Macedonia
Mediha Selimi PHI General Hospital Gostivar- Department of Internal Medicine, North Macedonia
Vladimir Mitreski PHI Institute of lung disease and tuberculosis, Faculty of Medicine, Ss.Cyril and Methodius University in Skopje, North Macedonia
Nikola Chamurovski PHI Institute of lung disease and tuberculosis, Faculty of Medicine, Ss.Cyril and Methodius University in Skopje, North Macedonia
Maja Jakimovska Dimitrovska University Institute of Radiology, Faculty of Medicine, Ss.Cyril and Methodius University in Skopje, Republic of North Macedonia

Abstract

The aims of this study are: to compare diagnostic values and laboratory variables of alanine aminopeptidase (microsomal AAP), Î³-glutamyl transferase (Î³-GT), Î²2-microglobulin (Î²2-M), C-Reactive Protein (CRP), and the index for disease activity (PASI) in the early diagnosis of previously untreated psoriatic arthritis (Psa) and to see how untreated psoriatic arthritis affected tubular function and the sensitivity of the brush border region, together with the diagnostic value of enzymes originating from the proximal renal tubules. From the standard methods of the International Federation for Clinical Chemistry (IFCC), we used the kinetic method for the determination of alanine aminopeptidase (microsomal AAP), Î³-glutamyl transferase ((Î³-G) and MEIA (Microparticles Enzyme Immunoassay (Abbott A_xsym system)) for determination of Î²2microglobulin in the urine. We examined samples (serum and urine) from 70 participants (35 Psa untreated, 35 healthy control group). RF and CRP were determined with the latex agglutination test in the same participants. Of the 35 examined patients with Psa, 12 (34.28%) showed presence of APP enzymuria, 8 patients (22.85%) showed presence of Î³-GT, and a low percentage (0% showed presence of Î²2-microglobulin in urine. AAP has a higher sensitivity thanÎ³-GT and Î²2-M in asymptomatic renal lesions in untreated Psa.

Keywords: alanine aminopeptidase (AAP),Î³-GT (Î³-glutamyl transferase),Î²2-microglobulin (Î²2-M), spondyloarthropathies.

https://doi.org/10.55302/JMS2361087s

References

1. Helliwell PS, Taylor WJ. Classification and diagnostic criteria for psoriatic arthritis. Ann Rheum Dis2005;64Suppl 2:ii3â€“ii8.
2. Mueller PW. Detecting the renal effects of cadmium toxicity. Clin Chem 1993;39:743-745.
3. Schmitt J, Wozel G. The psoriasis area and severity index is the adequate criteria to define severity in chronic plaque-type psoriasis. Dermatol2005;210:194-199.
4. Pipitone N, Kingsley GH, Manzo A, Scott DL, Pitzalis C. Current concepts and new developments in the treatment of psoriatic arthritis. Rheumatology2003;42:1138-1148.
5. McHugh NJ, Balachrishnan C, Jones SM. Progression of peripheral joint disease in psoriatic arthritis: a 5-yr prospective study. Rheumatology Oxford2003;42:778-783.
6. Palazzi C, Olivieri I, Petricca A, Salvarani C. Rheumatoid arthritis or psoriatic symmetric polyarthritis? A difficult differential diagnosis. ClinExpRheumatol2002;20:3-4.
7. Helliwell PS. Relationship of psoriatic arthritis with the other spondyloarthropathies. CurrOpinRheumatol2004;16:344-349.
8. Alenius GM, Berglin E, Dahlqvist SR. Antibodies against cyclic citrullinated peptide (CCP) in psoriatic patients with or without joint inflammation. Ann Rheum Dis2006;65:398-400.
9. Korendowych E, Owen P, Ravindran J, Carmichael C, McHugh N. The clinical and genetic associations of anti-cyclic citrullinated peptide antibodies in psoriatic arthritis. Rheumatology (Oxford) 2005;44:1056-1060.
10. Inanc N, Dalkilic E, Kamali S, Kasapoglu-Gunal E, Elbir Y, Direskeneli H, Inanc M. Anti-CCP antibodies in rheumatoid arthritis and psoriatic arthritis. Clin Rheumatol 2007;26:17-23.
11. Abdel Fattah NSA, Hassan HE, Galal ZA. Antibodies to cyclic citrullinated peptides in patients with psoriatic arthritis. Egypt J Dermatol Venereol2008;28:13-23.