TYPE OF CYP450 2C19 GENE METABOLIZERS IN PATIENTS UNDERGOING PERCUTANEOUS CORONARY INTERVENTION IN THE REPUBLIC OF NORTH MACEDONIA -A PILOT STUDY

  • Tamara Savevska Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Olivija Efinska Mladenovska Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Kristina Stamatovska Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Magdalena Otljanska PHI University Clinic for cardiology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Frosina Arnaudova PHI University Clinic for cardiology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Matej Otljanski PHI University Clinic for cardiology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Meri Kirijas Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Teodora Brnjarchenska Blazhevska Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Olgica Sibinovska Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Gorjan Milanovski Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Stefani Iljoska
  • Boban Dobrevski Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Todor Arsov Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Aleksandat Petlichkovski Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia
  • Dejan Kiril Trajkov Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia

Abstract

Clopidogrel has an antiplatelet effect, which is used to reduce the risk of myocardial infarction and stroke in high-risk patients in the context of primary prophylaxis. In the body it is subjected to metabolism by the liver enzyme system CYP450. In this project, laboratory conditions have been established to determine the polymorphisms of the CYP450 2C19 gene. The structure of the polymorphisms in the CYP450 2C19 gene and the type of metabolizer has been determined.This study included 42 heart patients with St. post PCI, mean age 65-9.7 years, treated with 75 mg Clopidogrel daily. The following polymorphisms were examined: c.681G> A; c.636G> A; c.1A> G; c.1297C> T; c.395G> A; c.819 + 2T> A; c.358T> C; c.-806C> T. Appropriate laboratory conditions have been established for the precise determination of polymorphisms in the CYP450 2C19 gene and the determination of the type of metabolizer. The results showed that in our patients the largest number of polymorphisms occur at the position c.681G> A which leads to the creation of an intermediate metabolizer, while the polymorphism at the position c.-806C> T leads to the creation of an ultrafast metabolite. The largest number of polymorphisms occurs in c.681G> A CYP450 2C19 gene, which leads to the creation of an intermediate metabolizer. Because of this, and before starting the therapy in patients St. post PCI, it is necessary to first determine what clopidogrel metabolizers they are and whether they possess LF or GF alleles.


Key words: clopidogrel, metabolite, polymorphisms, CYP450 2C19 gene.

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Published
2022-12-29
How to Cite
SAVEVSKA, Tamara et al. TYPE OF CYP450 2C19 GENE METABOLIZERS IN PATIENTS UNDERGOING PERCUTANEOUS CORONARY INTERVENTION IN THE REPUBLIC OF NORTH MACEDONIA -A PILOT STUDY. Journal of Morphological Sciences, [S.l.], v. 5, n. 3, p. 78-84, dec. 2022. ISSN 2545-4706. Available at: <https://jms.mk/jms/article/view/vol5no3-13>. Date accessed: 09 feb. 2023.
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Articles