ASSOCIATION OF NOS3 GENE POLYMORPHISM rs1799983 WITH IN-STENT RESTENOSIS OF CORONARY ARTERIES

  • Zan Zimbakov University Clinic of Cardiology, Clinical Centre “Mother Theresa”, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, R. North Macedonia
  • Oliver Busljetik University Clinic of Cardiology, Clinical Centre “Mother Theresa”, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, R of North Macedonia
  • Silvana Jovanova University Clinic of Cardiology, Clinical Centre “Mother Theresa”, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, R. North Macedonia

Abstract

Revascularization by percutaneous coronary intervention (PCI) with stent implantation is an invasive technique commonly used in the therapy of acute coronary syndromes, including the myocardial infarction and other types of ischemic coronary disease. Although it is highly effective, some patients develop an in-stent restenosis requiring repeat percutaneous revascularization. Recent studies have indicated genetic association of single nucleotide polymorphisms in certain genes with the risk of restenosis. The aim of this study was to investigate the association of NOS3 gene rs1799983 (Glu298Asp) polymorphism with the in-stent restenosis of implanted coronary stents. In this prospective, observational, genetic-associated, case-control study demographic, clinical and laboratory data were analyzed from preliminary selected group of 77 patients with implanted coronary stents, of which 46 were with in-stent restenosis and 31 without restenosis. The gender and age distributions were similar among the two patient groups. Using different genetic models, genetic association of NOS3 gene rs1799983 polymorphism and in-stent restenosis was revealed (p<0.05). Restenosis odds ratio indicated the protective role of the dominant G allele, while the minor variant T allele was associated with an increased risk of in-stent restenosis.


Keywords: in-stent restenosis, coronary stent, polymorphism gene, NOS3.


https://doi.org/10.55302/JMS2251096z

References

1. Schmidt T, Abbott JD. Coronary Stents: History, Design, and Construction. J Clin Med. 2018; 7(6). pii: E126. doi: 10.3390/jcm7060126.
2. Dangas GD, Claessen BE, Caixeta A, Sanidas EA, Mintz GS, Mehran R. In-stent restenosis in the drug-eluting stent era. J Am Coll Cardiol. 2010; 56(23):1897-907. doi: 10.1016/j.jacc.2010.07.028.
3. Buccheri D, Piraino D, Andolina G, Cortese B. Understanding and managing in-stent restenosis: a review of clinical data, from pathogenesis to treatment. J Thorac Dis. 2016; 8(10):E1150-E1162. doi: 10.21037/jtd.2016.10.93.
4. Alfonso F, Byrne RA, Rivero F, Kastrati A. Current treatment of in-stent restenosis. J Am Coll Cardiol. 2014; 63(24):2659-73. doi: 10.1016/j.jacc.2014.02.545.
5. Alraies MC, Darmoch F, Tummala R, Waksman R. Diagnosis and management challenges of in-stent restenosis in coronary arteries. World J Cardiol. 2017; 9(8):640-651. doi: 10.4330/wjc.v9.i8.640.
6. Garg UC, Hassid A. Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells. J Clin Invest. 1989; 83(5):1774-7.
7. Veldman BA, Spiering W, Doevendans PA, Vervoort G, Kroon AA, de Leeuw PW, Smits P. The Glu298Asp polymorphism of the NOS 3 gene as a determinant of the baseline production of nitric oxide. J Hypertens. 2002; 20(10):2023-7.
8. Gomma AH, Elrayess MA, Knight CJ, Hawe E, Fox KM, Humphries SE. The endothelial nitric oxide synthase (Glu298Asp and -786T>C) gene polymorphisms are associated with coronary in-stent restenosis. Eur Heart J. 2002; 23(24):1955-62.
9. Suzuki T, Okumura K, Sone T, Kosokabe T, Tsuboi H, Kondo J, Mukawa H, Kamiya H, Tomida T, Imai H, Matsui H, Hayakawa T. The Glu298Asp polymorphism in endothelial nitric oxide synthase gene is associated with coronary in-stent restenosis. Int J Cardiol. 2002; 86(1):71-6.
10. Shuvalova YA, Kaminnyi AI, Meshkov AN, Shirokov RO, Samko AN. Association between polymorphisms of eNOS and GPx-1 genes, activity of free-radical processes and in-stent restenosis. Mol Cell Biochem. 2012; 370(1-2):241-9. doi: 10.1007/s11010-012-1419-3.
11. Zholdybayeva EV, Talzhanov YA, Aitkulova AM, Tarlykov PV, Kulmambetova GN, Iskakova AN, Dzholdasbekova AU, Visternichan OA, Taizhanova DZh, Ramanculov YM. Genetic risk factors for restenosis after percutaneous coronary intervention in Kazakh population. Hum Genomics. 2016; 10(1):15. doi: 10.1186/s40246-016-0077-z.
12. Zeng WP, Zhang R, Li R, Luo JF, Hu XF. Association of the Endothelial Nitric Oxide Synthase Gene T786C Polymorphism with In-Stent Restenosis in Chinese Han Patients with Coronary Artery Disease Treated with Drug-Eluting Stent. PLoS One. 2017; 12(1):e0170964. doi: 10.1371/journal.pone.0170964.
Published
2022-05-04
How to Cite
ZIMBAKOV, Zan; BUSLJETIK, Oliver; JOVANOVA, Silvana. ASSOCIATION OF NOS3 GENE POLYMORPHISM rs1799983 WITH IN-STENT RESTENOSIS OF CORONARY ARTERIES. Journal of Morphological Sciences, [S.l.], v. 5, n. 1, p. 96-102, may 2022. ISSN 2545-4706. Available at: <https://jms.mk/jms/article/view/vol5no1-13>. Date accessed: 25 june 2022.
Section
Articles