TIME-DEPENDENT EXPRESSION OF IL-1Î² mRNA - A PRELIMINARY STUDY  FOR POSSIBLE WOUND AGE MARKER

Natasha Bitoljanu; Ana Ivcheva; Viktorija Belakaposka Srpanova; Ljupco Cakar; Rosica Siamkouri; Aleksandar Stankov; Goran Pavlovski; Sasho Risteski; Zlatko Jakovski

Natasha Bitoljanu Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Ana Ivcheva Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Viktorija Belakaposka Srpanova Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Ljupco Cakar Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Rosica Siamkouri Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Aleksandar Stankov Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Goran Pavlovski Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Sasho Risteski Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia
Zlatko Jakovski Institute Forensic Medicine, Criminalistics and Medical Deontology, Faculty of Medicine, Ss Cyril and Methodius University in Skopje, North Macedonia

Abstract

Accurate estimation of wound age is a very important task in forensic medicine field, because it can helps in reconstruction of crime scene. IL-1Î² is proinflamatory cytokine and plays a major role in wound healing process. The aim of the study is to develop a method for the reliable estimation of dermal injury age. In this study the expression of IL-1Î² mRNA was evaluated by real-time PCR at skin human wounds with different post-injuried period. We collected 27 human skin wound samples from forensic autopsy cases at our Institute. In our study were include samples from lacerations, incised wounds and stab wounds, with a known time of injury and death. Wound samples were divided into five groups: control group (n=5); first group consisted of cases with immediate death (n=7); second group consisted of cases with survival from 1 hour to 6 hours (n=5); the third group comprised cases with survival from 6 hours to 72 hours (n=3); the fourth group comprised cases with survival from 72 hours to 168 hours (n=7). In this study we detected increased expression levels of IL-1 Î² mRNA in the second group with survival time from 1 to 6 hours.

Keywords: IL-1Î²; wound age; Real-time PCR; autopsy cases; human dermal injuries.

https://doi.org/10.55302/JMS2361121b

References

1. Wang Y, Yamamoto Y, Kuninaka Y, Kondo T, Furukawa F. Forensic potential of MMPs and CC chemokines for wound age determination, J, Forensic Sci. 2015; 60:6.
2. Wang L.L. et al. A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation. Journal of Forensic and Legal Medicine. 2016; 39: 138-146.
3. Birincioglu I, Akbaba M, Alver A, Kul S, Ozer E, Turan N, Senturk A, Ince I. Determination of skin wound age by using cytokines as potential markers. Journal Of Forensic and Legal Medicine. 2016; 44: 14-19.
4. Du Qiu-xiang, Wang Xiao-wei, Zhang Lei et al. Relative expression of indicators for wound age estimation in forensic pathology, Journal of Forensic Medicine, April 2015, Vol.31, No 2.
5. Khalaf A.A., Hassanen E.I., Zaki A.R., Tohamy A.F., Ibrahim A.A. Histopathological, imunohistochemical and molecular studies for determination of wound age and vitality in rats, Int Wound J. 2019:1-10.
6. Na Li, Qiuxiang Du, Rufeng Bai, Junhong Sun. Vitality and wound age estimation in forensic pathology: review and future prospects. Forensic sciences research. 2020; 5: 15-24.
7. Palagummi S. et al. A multiplex analysis of RNA expression during injury healing in human dermal injuries for injury age estimation. Forensic science International: Genetics Supplement Series. 2013; 4: e 17-e18.
8. Grellner W., Madea, B. Demands on scientific studies: vitality of wounds and wound age estimation. Forensic Science International. 2007; 165 (2-3): 150-154.
9. Velnar T. et al. The wound healing process: an overview of the cellular and molecular mechanisms. The Journal of International Medical research. 2009; 37:1528-1542.
10. Grazul-Bilska A. et al. Wound healing: The role of growth factors. Drugs of today. 2003; 39 (10): 787-800
11. Barrientos, S., Stojadinovic, O., Golinko, M., Brem, H., & Tomic-Canic, M. Growth factors and cytokines in wound healing. Wound Repair and Regeneration. 2008; 16(5): 585-601.
12. Kondo, T., & Ishida, Y. Molecular pathology of wound healing. Forensic Science International. 2010; 203: 93-98.
13. Zhang JM, An J. Cytokines, Inflammation and Pain. Int. Anesthesiol. Clin. 2007; 45 (2):27-37.
14. Betz P. Pathophysiology of wound healing. Chapter 17. In: James JP, Busuttil A, Smock W, eds. Forensic Medicine Clinical and Pathological Aspects. 2003: 83-89. London.
15. Kondo T, Ohshima T. the dynamics of inflammatory cytokines in the healing process of mouse skin wound: a preliminary study for possible wound age determination. Int J J Leg Med. 1996; 108: 231-236.
16. Hu J at al. The role of interleukin â€“ 1 in wound biology: Part II: In vivo and human translation studies. AnesthAnalg. 2010; 111(6): 1534-42.
17. https://www.oncotarget.com/article/22550/text/.
18. https://jhoonline.biomedcentral.com/articles/10.1186/s13045-016-0269-y.
19. Sun J-h, Zhu X-y, Dong T-n et al. An â€œup, no change, or downâ€ system: time-dependent expression of mRNAs in contused skeletal muscle of rats used for wound age estimation. Forensic Sci Int. 2017; 272:104â€“110.
20. Fronczek J, Lulf R, Korkmaz HI et al. Analysis of infammatory cells and mediators in skin wound biopsies to determine wound age in living subjects in forensic medicine. Forensic Sci Int. 2015; 247:7â€“13.
21. HernÃ¡ndez-Cueto C, Girela E, Sweet DJ. Advances in the diagnosis of wound vitality: a review. Am J Forensic Med Pathol. 2000; 21:21â€“31.
22. Yu T-S, Cheng Z-H, Li L-Q et al. The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats. Int J Legal Med. 2010; 124:397â€“404.
23. Kondo, T., & Ohshima, T. The dynamics of inflammatory cytokines in the healing process of mouse skin wound: A preliminary study for possible wound age determination. International Journal of Legal Medicine. 1996; 108:231â€“236.
24. Takamiya, M., Biwasaka, H., Saigusa, K., Nakayashiki, N., & Aoki, Y. Wound age estimation by simultaneous detection of 9 cytokines in human dermal wounds with a multiplex bead-based immunoassay: an estimative method using outsourced examinations. Legal Medicine. 2009; 11(4), 186-190.
25. Sato, Y., & Ohshima, T. The expression of mRNA of proinflammatory cytokines during skin wound healing in mice: a preliminary study for forensic wound age estimation (II). International Journal of Legal Medicine. 2000; 113(3), 140-145.