HUMORAL IMMUNE RESPONSE BEFORE AND AFTER SURGICAL THERAPY IN PATIENTS WITH ODONTOGENIC INFLAMMATORY  CYSTS

Irena Stojanova; Oliver Dimitrovski; Mirjana Markovska Arsovska; Muhamet Bajrami; Bruno Nikolovski

Irena Stojanova Department of Oral Surgery and Implantology, University Dental Clinical Center “St. Pantelejmon”, Skopje, North Macedonia
Oliver Dimitrovski Department of Oral Surgery and Implantology, University Dental Clinical Center “St. Pantelejmon”, Skopje, North Macedonia
Mirjana Markovska Arsovska Department of Oral Surgery and Implantology, University Dental Clinical Center “St. Pantelejmon”, Skopje, North Macedonia
Muhamet Bajrami Department of Oral Surgery and Implantology, University Dental Clinical Center “St. Pantelejmon”, Skopje, North Macedonia
Bruno Nikolovski Department of Oral Surgery and Implantology, University Dental Clinical Center “St. Pantelejmon”, Skopje, North Macedonia

Abstract

Odontogenic inflammatory cysts are pathological lesions that are often represented in clinical practice and they presented potential focal points with an impact on other organs and systems in the body. The aim of this research is to compare the patients’ humoral immune response by verifying the level of immunoglobulins IgA, IgG and IgM in the serum before and one month after surgical therapy in patients with odontogenic inflammatory cysts. 44 male and female patients diagnosed with odontogenic inflammatory cysts were included in the study and divided into three groups (Group 1-patients with radicular cysts-23, Group 2-patients with residual cysts-10 and, Group 3-patients with periodontal cysts-11). Тhe values of IgA, IgM and IgG in serum werе examined before and one month after the surgical therapy in the device Cobas 6000 model c501 (Roche, Germany). In all three examined parameters of humoral immunity (IgA, IgG and IgM in serum), a significant difference was observed in the patients in addition to a decrease in their values 1 month after the surgical intervention compared to before the intervention. The level of IgA before and 1 month after intervention between the three groups of patients did not indicate a significant difference for a consequent p=0.2716 vs. p=0.2898. A significant difference between the three groups of patients, before and 1 month after the surgery, was also not found in terms of the IgG level for a consequent p=0.2692 vs. p=0.3614. The comparison of the three groups of patients regarding the level of IgM in serum indicated a significantly higher value of this parameter in patients with periodontal cyst compared to radicular and residual cyst before the intervention (p=0.0067) and 1 month after the intervention (p=0.0263). The levels of immunoglobulins in patients with odontogenic inflammatory cysts before surgical treatment were significantly elevated, depending on the type of the cyst. Their surgical removal generate a decrease in their level. These findings suggest that IgA, IgG and IgM may play an important role in the occurrence, development and persistence of cystic lesions.

Keywords: Odontogenic inflammatory cysts, immunological analysis, humoral immune response, immunoglobulins IgA IgG and IgM.

References

1. Can Apical Periodontitis Modify Systemic Levelsof Inﬂammatory Markers? A Systematic Reviewand Meta-analysisMaximiliano Schunke Gomes,Trevor CharlesBlattner,Manoel Sant’Ana Filho,Fabiana Soares Grecca,,Fernando Neves Hugo,Ashraf F. Fouad, MS,‡and Mark A. Reynolds, JOE — Vol.39, Num 10, October 2013 (1205-1217).
2. Inflammatory odontogenic cysts, a briefliterature review Bruna CastilhoCanassa, Angelo Jose Pavan, Journal of surgical and clinical dentistry JSCD,2014 (20-28).
3. CD1a-positive cells in odontogenic cysts. Piattelli A, Rubini C, Iezzi G, Fioroni M. Journal of Endodontics, 2002;28, (267-268).
4. Radicular Cyst: An Update of the Biological Factors Related to Lining Epithelium reviw article, Lisiane Bernardi, Fernanda Visioli, Carolina Nor, BPharm and Pantelis Varvaki Rados, Journal of Endodontics 2015, (9-10).
5.Cystic Lesions of the Jaws – A Clinicopathological Study of 322 Cases and Review of the Literature Esther Manor 1, Leonid Кachko2, Max B. Puterman3, George Szabo 4, Lipa Bodner 5, International Journal of Med. Sci. 2012 (20-26).
6. The World Health Organization Classification of Odontogenic Lesions: A Summary of the Changes of the 2017 (4th) Edition Merva Soluk Tekkenesini, John M. Wright2, Turkish Journal of Pathology 2017 (12-15).
7. Atypical presentation of lateral periodontal cyst associated with impacted teeth: two case reportsS. Buchholzer1†, Fabien Bornert, D. Di Donna and T. Lombardi, Buchholzer et al. BMC Oral Health ,2021 (21-28).
8. The inflammatory paradental cyst: A critical review of 342 cases from a literature survey, Philipsen HP, Reichart PA, Ogawa I, Suei Y, Takata T Journal of Oral Pathology Med.2004;(147-155).
9. Validation of methods performance for routine biochemistry analytes at Cobas 6000 analyzer series module c 501, Biochemia Medica 2011, (82-90).
10. Radiological, histological and immunohistochemical evaluation of periapical inflammatory lesions Antonela Marcela Erar, Cosmina Ioana Ondo r, Luminita Matros, Radu Eptimu Ampian, Romanian Journal of Morphology and Embryology 2016 (419-425).
11. Clinicopathological Evaluation of Odontogenic Jaw Cysts. Dr. Mohammad kamrujjamana, Dr. SajidHasan, Dr. A.S.M. Didar Alam Khan c, Dr Hasan Tareq Bin Noor, Dr. Abul Hasnat, Update Dental College Journal 2015 (231-236).
12. Association between Endodontic Infection, Its Treatment and Systemic Health: A Narrative Review- Sadia Ambreen Niazi 1,andAbdulaziz Bakhsh 2,Medicina 2022, (58-65).
13. Influence of odontogenic cysts on immune system – cytometric analysis of T lymphocytes subpopulation Mansur Rahnama1, Izabela Jastrzębska-Jamrogiewiczt,Rafał Jamrogiewicz1, Janusz Kocki2, Polish Annals of Medicine. 2018, (231–236).
14. The Levels of Serum Immunoglobulin A, G and M in Oral Inflammatory Cysts before and after Surgical Therapy, Oliver Dimitrovski, Vancho Spirov, Blagoja Dastevski, Filip Koneski 10.2478/Balcan journal of dental medicine,2018 (81-86).
15. Kubota Y, Ninomiya T, Oka S, Takenoshita Y, Shirasuna K. Interleukin-1alpha-dependent regulation of matrix metalloproteinase-9 (MMP-9) secretion and activation in the epithelial cells of odontogenic jaw cysts. J Dent Res, 2000,(1423-1430.).