• Serge Pavlov
  • Marina Kumetchko Kharkiv Medical Academy of Postgraduate Education
  • Olga Litvinova
  • Nataliia Babenko
  • Nikita Valilshchykov
  • Natalia Semko
  • Odday Shamas


Concomitant diseases can cause a violation of the orderly and timely course of the wound healing process, which dramatically increases the risk of chronic wounds. Currently, there are only a small number of studies studying the relationship between chronic kidney disease and impaired wound healing. The aim of our work was to study the peculiarities of the morphological changes of the skin during the healing of wounds complicated by chronic kidney disease in the experiment.

Studies were performed on 48 white rats. Animals were divided into two groups: control and experimental (rats with chronic kidney disease (CKD)). A model of a chronic wound was reproduced in animals of both groups. On the 7th, 15th and 28th days after the application of wounds, 6 rats from each group were removed from the experiment. Histological examination were subject to skin samples of animals of both groups. To assess the dynamics of wound area reduction, digital macro photography of the wound surface was performed.

The results of histological studies indicate an increase in the time course of the phases of inflammation, as well as the imposition of phases on each other. Measurement of the area of the wound surface revealed a slowdown in the process of reducing the area of wounds at all stages of healing in animals with CKD compared with intact animals. Differences in the structure of the tissue are detected even after the completion of the wound healing process in both groups.

The results of our study confirm the significant effect of CKD on wound healing. A prolongation of the inflammatory stage of healing is observed, which leads to disruption of the normal epithelialization of the wound and slower maturation of granulation tissue in experimental animals.


1. Houreld NN, Ayuk SM, Abrahamse H. Cell adhesion molecules are mediated by photobiomodulation at 660
nm in diabetic wounded fibroblast cells. Cells 2018; 7(4):30.
2. Seth AK, De la Garza M, Fang RC, Hong SJ, Galiano RD. Excisional wound healing is delayed in a murine
model of chronic kidney disease. PLoS ONE 2013; 8(3): e59979.
3. Hill NR, Fatoba ST, Oke JL, Hirst JA, O’Callaghan CA, Lasserson DS, et al. Global prevalence of chronic kidney
disease – a systematic review and meta-analysis. PLoS ONE 2016; 11(7):e0158765.
4. Cheung AH, Wong LM. Surgical infections in patients with chronic renal failure. Infect Dis Clin North Am
2001; 15:775–96.
5. Shindo K, Kosaki G. Effects of chronic renal failure on wound healing in rats. II. Microscopic study and
hydroxyproline assay. Jpn J Surg 1982; 12:46–51.
6. Yue DK, McLennan S, Marsh M, Mai YW, Spaliviero J, Delbridge L, et al. Effects of experimental diabetes,
uremia, and malnutrition on wound healing. Diabetes 1987; 36:295–9.
7. Vlahovic P, Cvetkovic T, Savic V, Stefanovic V. Dietary curcumin does not protect kidney in glycerol-induced
acute renal failure. Food Chem Toxicol 2007; 45(9):1777–82.
8. Kondakov II, Topchii II, Kirienko OM. Influence of glicerol on functional-morphological indicators of kidneys
at modelling renal insufficiency in rats. Ukr J Nephrol Dialysis 2013; 3(39):14-20.
9. Zinatullin RM, Gizatullin TR, Pavlov VN, Kataev VA, Farhutdinov RR, Baymurzina JL, et al., inventor; Zinatullin
RM, Gizatullin TR, assignee. A method for modeling a trophic wound in an experiment. Russian patent RF
2510083. 2014; March 20.
10. Sarkisov DS, Perov JuL, eds. Mikroskopicheskaja tehnika: rukovodstvo dlja vrachej i laborantov. Moscow:
Medicina; 1996; 544 p.
11. Mousleh R, Al Laham S, Al-Manadili A. The preventive role of pioglitazone in glycerol-induced acute kidney
injury in rats during two different treatment periods. Iran J Med Sci 2018; 43(2):184-94.
12. Yurchenko TN, Kondakov II, Strona VI. Renal effects with the introduction of placental cryoextract on the
background of experimental renal failure Probl Cryobiol Cryomed 2014; 24(1):75–8.
13. Pavlov SB, Kumechko MV, Litvinova OB, Babenko NM, Goncharova AV. Bone regulatory mechanisms
destruction in experimental chronic kidney disease. Fiziol Zh 2016; 62(3):54-9.
14. Wynn TA, Vannella KM. Macrophages in tissue repair, regeneration, and fibrosis. Immunity 2016; 44(3):450–62.
15. Zhao R, Liang H, Clarke E, Jackson C, Xue M. Inflammation in chronic wounds. Int J Mol Sci 2016; 17(12):2085.
16. Enoch S, Price P. Cellular, molecular and biochemical differences in the pathophysiology of healing between
acute wounds, chronic wounds and wounds in the aged. World Wide Wounds [Internet]. 2004 [updated 2017
Jun 04; cited 2004 Aug]. Available from:
17. Nauta A, Gurtner G, Longaker M. Woundhealing and regenerative strategies. Oral Dis 2011; 17(6):541-9.
18. Schreml S, Szeimies RM, Prantl L, Landthaler M, Babilas P. Wound healing in the 21st century. J Am Acad
Dermatol 2010; 63(5):866–81.
19. Du J. The research advancement of fibroblast on diabetic non-healing skin wound. J Community Med 2018;
20. Mescher AL. Macrophages and fibroblasts during inflammation and tissue repair in models of organ
regeneration. Regeneration (Oxf) 2017; 4(2):39–53.
21. Honnegowda TM, Kumar P, Udupa EG, Kumar S, Kumar U, Rao P. Role of angiogenesis and angiogenic
factors in acute and chronic wound healing. Plast Aesthet Res 2015; 2:243-9
22. Tonnesen MG, Feng X, Clark RA. Angiogenesis in wound healing. J Investig Dermatol Symp Proc 2000;
How to Cite
PAVLOV, Serge et al. FEATURES OF MORPHOLOGICAL CHANGES IN THE SKIN DURING WOUND HEALING. Journal of Morphological Sciences, [S.l.], v. 2, n. 2, p. 22-30, dec. 2019. ISSN 2545-4706. Available at: <http://jms.mk/jms/article/view/66>. Date accessed: 19 jan. 2020.